Course Name Code Semester T+U Hours Credit ECTS
Molecular Biological Techniques BMM 525 0 3 + 0 3 6
Precondition Courses
Recommended Optional Courses
Course Language Turkish
Course Level yuksek_lisans
Course Type Optional
Course Coordinator Doç.Dr. GAMZE GÜNEY ESKİLER
Course Lecturers Doç.Dr. GAMZE GÜNEY ESKİLER,
Course Assistants
Course Category Field Proper Education
Course Objective

Learning Molecular Biological techniques at advanced level

Course Content

In the content of this course; Basic rules of working in the laboratory, Introduction of basic equipment used in the laboratory, Usage areas of basic equipment used in the laboratory, Basic solutions used in the laboratory, Preparation of solutions used in the laboratory, DNA isolation, RNA isolation, DNA and RNA quantification, Electrophoresis,  Types of electrophoresis, Definition of PCR and its application, Preparation of PCR reaction mix, Determination of PCR conditions, Gene expression analysis, Mutation analysis methods, DNA sequence analysis, Methods of determining the effective doses of therapeutic agents, Investigation of the molecular effects of therapeutic agents on cellular processes and methods of  cell death type.

# Course Learning Outcomes Teaching Methods Assessment Methods
1 Knows the basic devices used in the laboratory and their applications Lecture, Question-Answer, Discussion, Testing, Homework, Performance Task,
2 Gains knowledge of DNA and RNA quantification Discussion, Question-Answer, Lecture, Performance Task, Homework, Testing,
3 Have information about electrophoresis and its types Discussion, Question-Answer, Lecture, Performance Task, Homework, Testing,
4 Gains knowledge of advanced molecular analysis Discussion, Question-Answer, Lecture, Performance Task, Homework, Testing,
5 Discussion, Question-Answer, Lecture, Performance Task, Homework, Testing,
Week Course Topics Preliminary Preparation
1 Basic rules of working in the laboratory
2 Application of basic devices used in the laboratory
3 Basic solutions used in the laboratory and their preparation
4 DNA isolation
5 RNA isolation
6 Electrophoresis and its applications
7 Definition and application areas of PCR
8 Gene expression analysis
9 Mutation analysis
10 DNA sequence analysis methods
11 Methods of determining effective doses of therapeutic agents
12 Methods of the types of cell death caused by therapeutic agents
13 Methods for determining the molecular effects of therapeutic agents on cellular processes
14 Current molecular techniques
Resources
Course Notes
Course Resources

Molecular Biology / A.G. Mclennan
Moleküler Biyolojide Kullanılan Yöntemler / Güler Temizkan, Nazlı Arda

 

Order Program Outcomes Level of Contribution
1 2 3 4 5
1 An understanding of integrated knowledge of science and engineering in the biomedical issues. X
2 The capability to apply advanced mathematics to solve the problems at the interface of engineering and biology
3 The ability to carry out measurements on living systems and interpret the obtained data X
4 An ability to function on multi-disciplinary teams X
5 Addressing the problems associated with both living and non-living materials and systems X
6 Proficiency in the use of modern techniques, tools, procedures, and knowledge useful in the definition and solution of problems in biomedical engineering area. X
Evaluation System
Semester Studies Contribution Rate
1. Ara Sınav 40
1. Ödev 30
1. Performans Görevi (Uygulama) 30
Total 100
1. Yıl İçinin Başarıya 60
1. Final 40
Total 100
ECTS - Workload Activity Quantity Time (Hours) Total Workload (Hours)
Course Duration (Including the exam week: 16x Total course hours) 16 3 48
Hours for off-the-classroom study (Pre-study, practice) 16 2 32
Mid-terms 1 20 20
Assignment 1 15 15
Performance Task (Application) 1 15 15
Final examination 1 20 20
Total Workload 150
Total Workload / 25 (Hours) 6
dersAKTSKredisi 6