Course Name Code Semester T+U Hours Credit ECTS
Protein Purification and Charactherization KIM 543 0 3 + 0 3 6
Precondition Courses
Recommended Optional Courses
Course Language Turkish
Course Level yuksek_lisans
Course Type Optional
Course Coordinator Doç.Dr. GÜLNUR ARABACI
Course Lecturers Doç.Dr. GÜLNUR ARABACI,
Course Assistants
Course Category
Course Objective The purpose of the course is to aid understanding structures and functions of proteins which are involved with living organism and inform about how important proteins in medicine, biotechnology and biochemistry.
Course Content Introduction, strategy and aim of protein purifications, general methods, special methods in protein determinations, chromatographic methods, electrophoretic methods, chemical changes of proteins, determinations of C-terminus of proteins, amino acid analysis.
# Course Learning Outcomes Teaching Methods Assessment Methods
1 Identifies amino acids and structure of proteins. Lecture, Question-Answer, Testing, Homework,
2 Identifies the general methods used in protein purifications Lecture, Question-Answer, Testing, Homework,
3 Classifies chromatographic methods. Lecture, Question-Answer, Testing, Homework,
4 Interprets the changes in proteins Lecture, Question-Answer, Testing, Homework,
5 Questions the identifications of amino acids in protein structures Lecture, Question-Answer, Problem Solving, Testing, Homework,
6 Discusses the latest methods for determinations of protein structure and functions Lecture, Question-Answer, Testing, Homework,
Week Course Topics Preliminary Preparation
1 Strategy and aim of protein purification
2 General methods
3 Special methods in protein determination
4 Protein extractions and subcellular fractions
5 Ion exchange chromatography
6 Gel filtration chromatography
7 Hydrophobic attraction chromatography
8 Electrophoretic method used in protein purifications
9 MIDTERM EXAM
10 Chemical changes in enzymes
11 Immunologic methods
12 Affinity chromatography and protein crystallization
13 Chemical and enzymatic degradations of proteins
14 Analysis techniques of amino acids and C- and N-terminal of polypeptides
Resources
Course Notes
Course Resources
Order Program Outcomes Level of Contribution
1 2 3 4 5
1 In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice. X
1 In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice.
2 Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. X
2 Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. X
3 - Constructs a problem with his/her adviser and develops a solution method and by evaluating the results apply them if it is necessary in his/ her area. X
3 - Constructs a problem with his/her adviser and develops a solution method and by evaluating the results apply them if it is necessary in his/ her area. X
4 -Transfer the current developments and his/her studies to the other related or nonrelated groups as written, orally and visually.
4 -Transfer the current developments and his/her studies to the other related or nonrelated groups as written, orally and visually.
5 - Develops new strategic approach and produces solutions in unexpected and complicated situations in his or her area of practice. X
5 - Develops new strategic approach and produces solutions in unexpected and complicated situations in his or her area of practice. X
6 - Develops strategic and practice plans and evaluates the obtained results within the framework of quality process in his or her study area.
6 - Develops strategic and practice plans and evaluates the obtained results within the framework of quality process in his or her study area. X
7 - Has written communication ability in one of well known foreign languages (“European Language Portfolio Global Scale”, Level A1).
7 - Has written communication ability in one of well known foreign languages (“European Language Portfolio Global Scale”, Level A1). X
8 -Has knowledge for software and hardware about computers and uses data processing. X
8 -Has knowledge for software and hardware about computers and uses data processing. X
9 Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena.
9 Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena. X
10 - Applies his or her digested knowledge and problem solving ability in advanced studies.
Evaluation System
Semester Studies Contribution Rate
1. Ödev 20
1. Performans Görevi (Seminer) 20
1. Ara Sınav 60
Total 100
1. Yıl İçinin Başarıya 50
1. Final 50
Total 100
ECTS - Workload Activity Quantity Time (Hours) Total Workload (Hours)
Course Duration (Including the exam week: 16x Total course hours) 16 3 48
Hours for off-the-classroom study (Pre-study, practice) 16 3 48
Mid-terms 1 15 15
Assignment 1 10 10
Performance Task (Seminar) 1 10 10
Final examination 1 20 20
Quiz 1 10 10
Total Workload 161
Total Workload / 25 (Hours) 6.44
dersAKTSKredisi 6