| Course Name | Code | Semester | T+U Hours | Credit | ECTS |
|---|---|---|---|---|---|
| Recombinant Dna Technology | BIY 509 | 0 | 3 + 0 | 3 | 6 |
| Precondition Courses | |
| Recommended Optional Courses | |
| Course Language | Turkish |
| Course Level | yuksek_lisans |
| Course Type | Optional |
| Course Coordinator | Prof.Dr. HÜSEYİN AKSOY |
| Course Lecturers | |
| Course Assistants | |
| Course Category | Available Basic Education in the Field |
| Course Objective | In 1973, after the occurrence of replicated DNA is cut by enzymes, recombinant DNA technology today is rapidly advancing basic science, medicine, agriculture, forensic biology, and methods used in every field, and differs from developing with each passing day. All students who graduate in the field of modern biology, basic knowledge in this field should be |
| Course Content | In 1973, after the occurrence of replicated DNA is cut by enzymes, recombinant DNA technology today is rapidly advancing basic science, medicine, agriculture, forensic biology, and methods used in every field, and differs from developing with each passing day. All students who graduate in the field of modern biology, basic knowledge in this field should be. All of the following weeks, according to the contents of the course were completed. |
| # | Course Learning Outcomes | Teaching Methods | Assessment Methods |
|---|---|---|---|
| 1 | To learn the basic methods used in molecular biology | Lecture, Question-Answer, Discussion, | Testing, Oral Exam, Homework, |
| Week | Course Topics | Preliminary Preparation |
|---|---|---|
| 1 | Basic principles of molecular genetics | |
| 2 | DNA replication, RNA processing, protein synthesis | |
| 3 | The creation of gene libraries | |
| 4 | Screening methods for the synthesis of the desired gene | |
| 5 | Southern blotting | |
| 6 | RNA, cDNA cloning techniques and technologies, Northern blotting | |
| 7 | Genomic libraries | |
| 8 | Plasmids, bacteriophage vectors | |
| 9 | PCR cloning method, and variations | |
| 10 | Bacterial protein expression by Western blotting | |
| 11 | Yeast, baculovirus, mammalian expression systems | |
| 12 | Protein purification process | |
| 13 | Structural protein analysis methods, crystallography, NMR | |
| 14 | Results and Discussion |
| Resources | |
|---|---|
| Course Notes | Lewin B (2008) Genes IX, Jones and Bartlett Publishers, MA, USA<br>Alberts B et.al (2002) Hücrenin moleküler biyolojisi, 4. Baskı, Garland Science, TÜBA Yayınları. |
| Course Resources | |
| Order | Program Outcomes | Level of Contribution | |||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Providing methods of development and obtaining information methods at the specialist scale | X | |||||
| 2 | To be able to practice the subject | X | |||||
| 3 | Having a basic knowledge in related field literature | X | |||||
| 4 | Explain and discuss the results | X | |||||
| 5 | Identify original topics | X | |||||
| 6 | Has the ability to use his / her achievements in biology in interdisciplinary studies. | X | |||||
| 7 | Develops attitude towards project-based work. | X | |||||
| 8 | Academic and cultural accumulation contributes to the process of being information society. | X | |||||
| 9 | Can use computer and information technology at advanced level in accordance with its purposes. | X | |||||
| 10 | Develop a positive attitude towards life-long learning. | X | |||||
| 11 | Develops knowledge at the level of expertise in the relevant disciplines, based on biology level qualifications. | X | |||||
| 12 | It is an individual who is sensitive to universal and social values, who observes, investigates, produces, and has ethical values in the fields / practices in his / her field of study. | X | |||||
| Evaluation System | |
|---|---|
| Semester Studies | Contribution Rate |
| 1. Ara Sınav | 30 |
| 1. Kısa Sınav | 7 |
| 1. Ödev | 30 |
| 1. Performans Görevi (Seminer) | 25 |
| 2. Kısa Sınav | 8 |
| Total | 100 |
| 1. Yıl İçinin Başarıya | 50 |
| 1. Final | 50 |
| Total | 100 |
| ECTS - Workload Activity | Quantity | Time (Hours) | Total Workload (Hours) |
|---|---|---|---|
| Course Duration (Including the exam week: 16x Total course hours) | 16 | 3 | 48 |
| Hours for off-the-classroom study (Pre-study, practice) | 16 | 2 | 32 |
| Mid-terms | 1 | 15 | 15 |
| Assignment | 1 | 10 | 10 |
| Performance Task (Seminar) | 1 | 30 | 30 |
| Final examination | 1 | 10 | 10 |
| Total Workload | 145 | ||
| Total Workload / 25 (Hours) | 5.8 | ||
| dersAKTSKredisi | 6 | ||