| Ders Adı | Kodu | Yarıyıl | T+U Saat | Kredi | AKTS |
|---|---|---|---|---|---|
| Proteome Analysıs | KIM 643 | 0 | 3 + 0 | 3 | 6 |
| Ön Koşul Dersleri | |
| Önerilen Seçmeli Dersler | |
| Dersin Dili | Türkçe |
| Dersin Seviyesi | Doktora |
| Dersin Türü | Seçmeli |
| Dersin Koordinatörü | Prof.Dr. GÜLNUR ARABACI |
| Dersi Verenler | Prof.Dr. GÜLNUR ARABACI, |
| Dersin Yardımcıları | |
| Dersin Kategorisi | Diğer |
| Dersin Amacı | Help to understand and interpret proteome analysis used in the diagnosis of disease and the abnormal changes in cell level. Also, inform about how proteomic works in biochemsitry, medicine and biotechnology. |
| Dersin İçeriği | Introduction, proteomic research in general, two dimensional electrophoresis (2-D) in protein analysis, protein degradation by enzymes and separation of polypeptides by HPLC, analysis of peptides and proteins, bioinformatics in proteome analysis, model systems of proteome analysis. |
| # | Ders Öğrenme Çıktıları | Öğretim Yöntemleri | Ölçme Yöntemleri |
|---|---|---|---|
| 1 | Interprets two dimensional electrophoresis (2-D) used in proteome analysis. | Lecture, Question-Answer, | Testing, Homework, |
| 2 | Identifies blotting and immune dying | Lecture, Question-Answer, | Testing, Homework, |
| 3 | Interprets HPLC analysis of the peptides formed from proteins degradation | Lecture, Question-Answer, Problem Solving, | Testing, Homework, |
| 4 | Analizes the mass spectrometers of the peptides. | Lecture, Question-Answer, | Testing, Homework, |
| 5 | Identifies the model system of proteomics. | Lecture, Question-Answer, | Testing, Homework, |
| 6 | Questions the latest methods used in protein structures analysis | Lecture, Question-Answer, | Testing, Homework, |
| Hafta | Ders Konuları | Ön Hazırlık |
|---|---|---|
| 1 | General introduction of proteomic researche | |
| 2 | Two dimensional electrophoresis (2-D) in protein analysis | |
| 3 | Blotting and immune dying | |
| 4 | Protein degradations by enzymes and separations of polypeptides by HPLC | |
| 5 | Analysis of peptides and proteins | |
| 6 | Mass spectrum of peptides and proteins | |
| 7 | Post-translational modufications | |
| 8 | Bioinformatics in differential proteome analysis | |
| 9 | MIDTERM EXAM | |
| 10 | Cell cultures in proteome analysis | |
| 11 | Model systems of proteome analysis | |
| 12 | Applications of proteome analysis: determinations, identifications and characterizations of human tumor cells | |
| 13 | Stabile isotope marking in proteome analysis | |
| 14 | Proteomic applications and new technologies |
| Kaynaklar | |
|---|---|
| Ders Notu | |
| Ders Kaynakları | |
| Sıra | Program Çıktıları | Katkı Düzeyi | |||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice. | X | |||||
| 1 | In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice. | X | |||||
| 2 | Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. | X | |||||
| 2 | Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. | X | |||||
| 3 | Constructs a problem independently and solves the problem by proposing an experimental solution method and applies to the advance level research by evaluating the results. | X | |||||
| 3 | Constructs a problem independently and solves the problem by proposing an experimental solution method and applies to the advance level research by evaluating the results. | X | |||||
| 4 | Transfers systematically the current developments and his or her advance level studies to the other related or nonrelated groups as written, orally and visually. | X | |||||
| 4 | Transfers systematically the current developments and his or her advance level studies to the other related or nonrelated groups as written, orally and visually. | X | |||||
| 5 | Develops new strategic approach and produces solutions by taking responsibility in unexpected and complicated situations in his or her area of practice. | X | |||||
| 5 | Develops new strategic approach and produces solutions by taking responsibility in unexpected and complicated situations in his or her area of practice. | X | |||||
| 6 | Develops advance strategic, political and practice plans and evaluates the obtained results within the framework of quality process in his or her study area. | X | |||||
| 6 | Develops advance strategic, political and practice plans and evaluates the obtained results within the framework of quality process in his or her study area. | X | |||||
| 7 | - Has advance level oral or written communication ability in one of well known foreign language (“European Language Portfolio Global Scale”, Level B1). | X | |||||
| 7 | - Has advance level oral or written communication ability in one of well known foreign language (“European Language Portfolio Global Scale”, Level B1). | X | |||||
| 8 | Has knowledge for software and hardware about computers, uses information and communication technologies. | X | |||||
| 8 | Has knowledge for software and hardware about computers, uses information and communication technologies. | X | |||||
| 9 | Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena. | X | |||||
| 9 | Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena. | X | |||||
| 10 | Applies the digested knowledge and problem solving ability in advance level studies among the different disciplines. | ||||||
| 10 | Applies the digested knowledge and problem solving ability in advance level studies among the different disciplines. | ||||||
| # | Ders Öğrenme Çıktılarının Program Çıktılarına Katkısı | PÇ 1 | PÇ 1 | PÇ 2 | PÇ 2 | PÇ 3 | PÇ 3 | PÇ 4 | PÇ 4 | PÇ 5 | PÇ 5 | PÇ 6 | PÇ 6 | PÇ 7 | PÇ 7 | PÇ 8 | PÇ 8 | PÇ 9 | PÇ 9 | PÇ 10 | PÇ 10 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Interprets two dimensional electrophoresis (2-D) used in proteome analysis. | ||||||||||||||||||||
| 2 | Identifies blotting and immune dying | ||||||||||||||||||||
| 3 | Interprets HPLC analysis of the peptides formed from proteins degradation | ||||||||||||||||||||
| 4 | Analizes the mass spectrometers of the peptides. | ||||||||||||||||||||
| 5 | Identifies the model system of proteomics. | ||||||||||||||||||||
| 6 | Questions the latest methods used in protein structures analysis |
| Değerlendirme Sistemi | |
|---|---|
| Yarıyıl Çalışmaları | Katkı Oranı |
| 1. Ödev | 20 |
| 1. Performans Görevi (Seminer) | 20 |
| 1. Ara Sınav | 50 |
| 1. Kısa Sınav | 10 |
| Toplam | 100 |
| 1. Yıl İçinin Başarıya | 50 |
| 1. Final | 50 |
| Toplam | 100 |
| AKTS - İş Yükü Etkinlik | Sayı | Süre (Saat) | Toplam İş Yükü (Saat) |
|---|---|---|---|
| Course Duration (Including the exam week: 16x Total course hours) | 16 | 3 | 48 |
| Hours for off-the-classroom study (Pre-study, practice) | 16 | 3 | 48 |
| Mid-terms | 1 | 15 | 15 |
| Assignment | 1 | 10 | 10 |
| Performance Task (Seminar) | 1 | 10 | 10 |
| Final examination | 1 | 20 | 20 |
| Toplam İş Yükü | 151 | ||
| Toplam İş Yükü / 25 (Saat) | 6,04 | ||
| dersAKTSKredisi | 6 | ||