Course Name Code Semester T+U Hours Credit ECTS
Methods In Molecular Biology GDM 541 0 3 + 0 3 6
Precondition Courses
Recommended Optional Courses
Course Language Turkish
Course Level yuksek_lisans
Course Type Optional
Course Coordinator Doç.Dr. AYŞE AVCI
Course Lecturers Doç.Dr. AYŞE AVCI,
Course Assistants
Course Category
Course Objective Giving information about the principles and applicaions of the basic methods applied in a molecular biology laboratory
Course Content 1. Cell distruption methods
1.1. Physical and chemical cell distruption methods
1.2. Seperation and purification methods
2. Isolation anad analysis of DNA and RNA
2.1. Isolation methods of bacterai, fungal and plant DNA
2.2. Analysis of purified DNA
2.3. Isolation methods of bacterai, fungal and plant DNA
2.4. Analysis of purified RNA
3. Polymerase chain reaction (PCR)
3.1. Types of PCR
4. DNA sequencing
5. Gen cloning methods
6. Hybridization and blotting
7. Probs
8. Purification of proteins and SDS-PAGE analysis
# Course Learning Outcomes Teaching Methods Assessment Methods
1 Comprehends the principles of basic molecular analysis methods , , , , , , ,
2 Comprehends performing basic molecular analysis , , , , , , , ,
Week Course Topics Preliminary Preparation
1 Introduction to the molecular analysis
2 Physical and chemical cell distruption methods
3 Seperation and purification methods
4 Isolation of DNA
5 Analysis of DNA
6 Isolation of RNA
7 Analysis of RNA
8 Polymerase chain reaction (PCR)
9 MIDTERM
10 DNA sequence analysis
11 Gene clonning
12 Hybridization and blotting
13 Probs
14 Purification and analysis of proteins
Resources
Course Notes Ayse Avcı, Lecture Notes
Course Resources 1. Moleküler Biyoloji. PC Turner, AG McLennan, AD Bates, MRH White, Nobel Yayın Dağıtım. 2004
2. Moleküler Biyolojide kullanılan yöntemler. G. Temizkan, N.Arda, Nobel Tıp Kitabevleri, 2004
3. Methods in Biotechnology, H.P.Schmauder, Taylor and Francis, 1997
4. Food Biotechnology. G.S. Mittal, Technomic Publishing Co. 1992.
Order Program Outcomes Level of Contribution
1 2 3 4 5
1 In addition to expanding and making deeper the knowledge established on high school education in a same or different area, to be able to reach knowledge, evaluate and interpret it to be able to do practice. X
2 By utilizing incomplete or limited data in his or her study area, to be able to expand the knowledge by scientific methods and use them in scientific, social and ethical responsibility.
3 To be able to define a problem, propose a solution method, solve it, evaluate the results and apply if it is necessary in his or her area.
4 To be able to transfer systematically the current developments, his or her studies to the other people as written, orally and visually.
5 To be able to develop new strategic approach and produce solutions by taking responsibility in unexpected and complicated situations in his or her area of practice. X
6 To be able to have oral or written communication ability in one of well know foreign language (“European Language Portfolio Global Scale”, Level B2). X
7 To have knowledge about software and hardware about computers and be able to use and improve data processing and communication abilities.
8 To be able to watch scientific, social, and ethical values and teach and control them in the step of data collection, evaluation and announcement of them. X
9 The qualifications gaining for values of scientific and ethical in stages of data collection, interpretation, dissemination and in all professional activities,
10 To create awareness about new and emerging applications of the profession; when the necessary gain skills to investigate and learn X
11 To understand the social and environmental aspects of engineering practice, gain skills to adapt to the social environment
Evaluation System
Semester Studies Contribution Rate
1. Ödev 20
Total 20
1. Yıl İçinin Başarıya 50
1. Final 50
Total 100
ECTS - Workload Activity Quantity Time (Hours) Total Workload (Hours)
Course Duration (Including the exam week: 16x Total course hours) 16 3 48
Hours for off-the-classroom study (Pre-study, practice) 16 2 32
Mid-terms 1 15 15
Assignment 1 15 15
Performance Task (Seminar) 1 20 20
Final examination 1 20 20
Total Workload 150
Total Workload / 25 (Hours) 6
dersAKTSKredisi 6