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Ders Tanımı

Ders Kodu Yarıyıl T+U Saat Kredi AKTS
PROTEIN PURIFICATION AND CHARACTHERIZATION KIM 543 0 3 + 0 3 6
Ön Koşul Dersleri
Önerilen Seçmeli Dersler
Dersin Dili Türkçe
Dersin Seviyesi Yüksek Lisans
Dersin Türü SECMELI
Dersin Koordinatörü Doç.Dr. GÜLNUR ARABACI
Dersi Verenler
Dersin Yardımcıları
Dersin Kategorisi
Dersin Amacı
The purpose of the course is to aid understanding structures and functions of proteins which are involved with living organism and inform about how important proteins in medicine, biotechnology and biochemistry.
Dersin İçeriği
Introduction, strategy and aim of protein purifications, general methods, special methods in protein determinations, chromatographic methods, electrophoretic methods, chemical changes of proteins, determinations of C-terminus of proteins, amino acid analysis.
Dersin Öğrenme Çıktıları Öğretim Yöntemleri Ölçme Yöntemleri
1 - Identifies amino acids and structure of proteins. 1 - 2 - A - C -
2 - Identifies the general methods used in protein purifications 1 - 2 - A - C -
3 - Classifies chromatographic methods. 1 - 2 - A - C -
4 - Interprets the changes in proteins 1 - 2 - A - C -
5 - Questions the identifications of amino acids in protein structures 1 - 2 - 15 - A - C -
6 - Discusses the latest methods for determinations of protein structure and functions 1 - 2 - A - C -
Öğretim Yöntemleri: 1:Lecture 2:Question-Answer 15:Problem Solving
Ölçme Yöntemleri: A:Testing C:Homework

Ders Akışı

Hafta Konular ÖnHazırlık
1 Strategy and aim of protein purification
2 General methods
3 Special methods in protein determination
4 Protein extractions and subcellular fractions
5 Ion exchange chromatography
6 Gel filtration chromatography
7 Hydrophobic attraction chromatography
8 Electrophoretic method used in protein purifications
9 MIDTERM EXAM
10 Chemical changes in enzymes
11 Immunologic methods
12 Affinity chromatography and protein crystallization
13 Chemical and enzymatic degradations of proteins
14 Analysis techniques of amino acids and C- and N-terminal of polypeptides

Kaynaklar

Ders Notu
Ders Kaynakları

Döküman Paylaşımı


Dersin Program Çıktılarına Katkısı

No Program Öğrenme Çıktıları KatkıDüzeyi
1 2 3 4 5
1 In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice. X
2 Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. X
3 - Constructs a problem with his/her adviser and develops a solution method and by evaluating the results apply them if it is necessary in his/ her area. X
4 -Transfer the current developments and his/her studies to the other related or nonrelated groups as written, orally and visually.
5 - Develops new strategic approach and produces solutions in unexpected and complicated situations in his or her area of practice. X
6 - Develops strategic and practice plans and evaluates the obtained results within the framework of quality process in his or her study area. X
7 - Has written communication ability in one of well known foreign languages (“European Language Portfolio Global Scale”, Level A1).
8 -Has knowledge for software and hardware about computers and uses data processing. X
9 Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena.
10 In addition to expanding and making deeper the knowledge established on undergraduate education in a same or different area, reaches the knowledge by doing research and also improves his or her knowledge to the expertise level by doing evaluation and practice.
11 Utilizing incomplete or limited data in his or her study area expands the knowledge by experimental methods and use those obtained knowledge by scientific, social and ethical responsibility. X
12 - Constructs a problem with his/her adviser and develops a solution method and by evaluating the results apply them if it is necessary in his/ her area. X
13 -Transfer the current developments and his/her studies to the other related or nonrelated groups as written, orally and visually.
14 - Develops new strategic approach and produces solutions in unexpected and complicated situations in his or her area of practice. X
15 - Develops strategic and practice plans and evaluates the obtained results within the framework of quality process in his or her study area.
16 - Has written communication ability in one of well known foreign languages (“European Language Portfolio Global Scale”, Level A1). X
17 -Has knowledge for software and hardware about computers and uses data processing. X
18 Protecting scientific, social, and ethical values, teaches and controls these values in the steps of collection, evaluation and announcement of data in the international arena. X
19 - Applies his or her digested knowledge and problem solving ability in advanced studies.

Değerlendirme Sistemi

YARIYIL İÇİ ÇALIŞMALARI SIRA KATKI YÜZDESİ
AraSinav 1 70
Odev 1 15
PerformansGoreviSeminer 1 15
Toplam 100
Yıliçinin Başarıya Oranı 50
Finalin Başarıya Oranı 50
Toplam 100

AKTS - İş Yükü

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