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Ders Tanımı

Ders Kodu Yarıyıl T+U Saat Kredi AKTS
METHODS İN MOLECULAR BİOLOGY GDM 541 0 3 + 0 3 6
Ön Koşul Dersleri
Önerilen Seçmeli Dersler
Dersin Dili Türkçe
Dersin Seviyesi Yüksek Lisans
Dersin Türü SECMELI
Dersin Koordinatörü Doç.Dr. AYŞE AVCI
Dersi Verenler
Dersin Yardımcıları
Dersin Kategorisi
Dersin Amacı
Giving information about the principles and applicaions of the basic methods applied in a molecular biology laboratory
Dersin İçeriği
1. Cell distruption methods
1.1. Physical and chemical cell distruption methods
1.2. Seperation and purification methods
2. Isolation anad analysis of DNA and RNA
2.1. Isolation methods of bacterai, fungal and plant DNA
2.2. Analysis of purified DNA
2.3. Isolation methods of bacterai, fungal and plant DNA
2.4. Analysis of purified RNA
3. Polymerase chain reaction (PCR)
3.1. Types of PCR
4. DNA sequencing
5. Gen cloning methods
6. Hybridization and blotting
7. Probs
8. Purification of proteins and SDS-PAGE analysis
Dersin Öğrenme Çıktıları Öğretim Yöntemleri Ölçme Yöntemleri
1 - Comprehends the principles of basic molecular analysis methods 1 - 2 - 3 - 5 - 13 - C - F -
2 - Comprehends performing basic molecular analysis 1 - 2 - 3 - 5 - 13 - 14 - C - F -
Öğretim Yöntemleri: 1:Lecture 2:Question-Answer 3:Discussion 5:Demonstration 13:Lab / Workshop 14:Self Study
Ölçme Yöntemleri: C:Homework F:Performance Task

Ders Akışı

Hafta Konular ÖnHazırlık
1 Introduction to the molecular analysis
2 Physical and chemical cell distruption methods
3 Seperation and purification methods
4 Isolation of DNA
5 Analysis of DNA
6 Isolation of RNA
7 Analysis of RNA
8 Polymerase chain reaction (PCR)
9 MIDTERM
10 DNA sequence analysis
11 Gene clonning
12 Hybridization and blotting
13 Probs
14 Purification and analysis of proteins

Kaynaklar

Ders Notu Ayse Avcı, Lecture Notes
Ders Kaynakları 1. Moleküler Biyoloji. PC Turner, AG McLennan, AD Bates, MRH White, Nobel Yayın Dağıtım. 2004
2. Moleküler Biyolojide kullanılan yöntemler. G. Temizkan, N.Arda, Nobel Tıp Kitabevleri, 2004
3. Methods in Biotechnology, H.P.Schmauder, Taylor and Francis, 1997
4. Food Biotechnology. G.S. Mittal, Technomic Publishing Co. 1992.

Döküman Paylaşımı


Dersin Program Çıktılarına Katkısı

No Program Öğrenme Çıktıları KatkıDüzeyi
1 2 3 4 5
1 In addition to expanding and making deeper the knowledge established on high school education in a same or different area, to be able to reach knowledge, evaluate and interpret it to be able to do practice. X
2 By utilizing incomplete or limited data in his or her study area, to be able to expand the knowledge by scientific methods and use them in scientific, social and ethical responsibility.
3 To be able to define a problem, propose a solution method, solve it, evaluate the results and apply if it is necessary in his or her area.
4 To be able to transfer systematically the current developments, his or her studies to the other people as written, orally and visually.
5 To be able to develop new strategic approach and produce solutions by taking responsibility in unexpected and complicated situations in his or her area of practice. X
6 To be able to have oral or written communication ability in one of well know foreign language (“European Language Portfolio Global Scale”, Level B2). X
7 To have knowledge about software and hardware about computers and be able to use and improve data processing and communication abilities.
8 To be able to watch scientific, social, and ethical values and teach and control them in the step of data collection, evaluation and announcement of them. X
9 The qualifications gaining for values of scientific and ethical in stages of data collection, interpretation, dissemination and in all professional activities,
10 To create awareness about new and emerging applications of the profession; when the necessary gain skills to investigate and learn X
11 To understand the social and environmental aspects of engineering practice, gain skills to adapt to the social environment

Değerlendirme Sistemi

YARIYIL İÇİ ÇALIŞMALARI SIRA KATKI YÜZDESİ
AraSinav 1 60
Odev 1 20
PerformansGoreviSeminer 1 20
Toplam 100
Yıliçinin Başarıya Oranı 50
Finalin Başarıya Oranı 50
Toplam 100

AKTS - İş Yükü

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